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Updated: Aug 12, 2026
Generation of Murine Monoclonal Antibodies by Hybridoma Technology
Published on: January 2, 2017
Subclass distribution of human myeloma proteins as determined with monoclonal antibodies
P Aucouturier1, J L Preud'Homme
1Laboratory of Immunology and Immunopathology (CNRS USA 1172), Poitiers University Hospital, France.
Subclasses of 176 IgG and 62 IgA myeloma proteins were determined by indirect ELISA with monoclonal antibodies, as well as by an immunoblotting technique (for monoclonal IgG) and by immunoelectrophoresis against the lectin jacalin (for IgA). The subclass distribution of monoclonal IgG did not reflect mean normal serum levels of the correspondent isotypes, with an over-representation of IgG1 and IgG4 and an under-representation of IgG2 and IgG3 in myeloma. Similarly, IgA2 myeloma were clearly under-represented.
Subclasses of 176 IgG and 62 IgA myeloma proteins were determined by indirect ELISA with monoclonal antibodies, as well as by an immunoblotting technique (for monoclonal IgG) and by immunoelectrophoresis against the lectin jacalin (for IgA). The subclass distribution of monoclonal IgG did not reflect mean normal serum levels of the correspondent isotypes, with an over-representation of IgG1 and IgG4 and an under-representation of IgG2 and IgG3 in myeloma. Similarly, IgA2 myeloma were clearly under-represented.
