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Murine B lymphoma cell lines release functionally active interleukin 2 after stimulation with Staphylococcus aureus
E Walker1, T Leemhuis, W Roeder
1Department of Medicine, Indiana University School of Medicine, Indianapolis 46223.
Journal of Immunology (Baltimore, Md. : 1950)
|February 1, 1988
Summary
Murine B cell tumor lines produce interleukin-2 (IL-2) after Staphylococcus aureus stimulation. This IL-2 activity is blocked by anti-IL-2 antibodies and detected via IL-2 mRNA expression.
Area of Science:
- Immunology
- Molecular Biology
Background:
- Murine B cell lines can produce lymphokine activity.
- Interleukin-2 (IL-2) is a key cytokine in immune responses.
Purpose of the Study:
- To investigate if murine B cell tumor lines produce IL-2.
- To characterize the nature of the lymphokine activity.
Main Methods:
- Stimulation of B cell lines (2PK-3, L10A2J) with Staphylococcus aureus.
- Blocking experiments using anti-IL-2 monoclonal antibodies (mAbs) and anti-IL-2 receptor (IL-2R) mAb.
- RNA-RNA hybridization to detect IL-2 mRNA.
Main Results:
- IL-2-like functional lymphokine activity was observed in stimulated B cell lines.
- This activity was blocked by anti-IL-2 and anti-IL-2R antibodies.
- Significant IL-2 mRNA levels were detected in stimulated B cells.
Conclusions:
- Murine B cell tumor lines synthesize and release IL-2.
- Staphylococcus aureus acts as a polyclonal activator for IL-2 production in these cells.