Related Experiment Video
Updated: Jan 23, 2026

A Protocol for Laboratory Housing of Turquoise Killifish Nothobranchius furzeri
Published on: April 11, 2018
Microsatellite instability diagnosis using the fully automated Idylla platform: feasibility study of an in-house
Laura Samaison1, Mélanie Grall1, Frédéric Staroz1
1Ouest Pathologie, Quimper, France.
Aim:
To study the performances of the Idylla MSI Assay in the diagnosis of microsatellite instability (MSI) or microsatellite stability (MSS).
Methods:
We selected 12 tumour samples previously tested for MSI focusing on cases with discrepant results between MLH1, PMS2, MSH2 and MSH6 immunohistochemistry and microsatellite molecular analyses (five cases) or doubtful immunohistochemistry (two cases). Idylla MSI Assay was compared with retrospective immunohistochemistry and molecular results.
Results:
Idylla MSI Assay showed an almost perfect concordance with microsatellite analysis results previously obtained (only one case with not fully conclusive analysis due to sample exhaustion). The full molecular analysis took less than 150 min per sample and revealed no mutation in any of the seven microsatellite sequences in five MSS samples and four to six mutated ones in seven MSI-High samples.
Conclusion:
At the era when the determination of MSI/MSS status is becoming important for rapid treatment choices, the Idylla MSI Assay consists of a valuable easy-to-perform diagnostic tool that allows, complementary to MLH1, PMS2, MSH2 and MSH6 immunohistochemistry, the diagnosis of MSI/MSS status in a single day.
More Related Videos
11:20Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology
Published on: January 24, 2018
05:57A Rapid Automated Protocol for Muscle Fiber Population Analysis in Rat Muscle Cross Sections Using Myosin Heavy Chain Immunohistochemistry
Published on: March 28, 2017
Related Concept Videos
Microtubule Instability
Serum Laboratory Studies, Stool Test, Breath Test
Immunocytochemistry and Immunohistochemistry
These...
Serum Studies: Renal Function Tests
Nursing Diagnosis
The nursing diagnosis focuses on evidence-based...
Molecular Models