Related Experiment Video
Updated: Jan 23, 2026

Mouse Body Temperature Measurement Using Infrared Thermometer During Passive Systemic Anaphylaxis and Food Allergy Evaluation
Published on: September 14, 2018
Microbiota therapy acts via a regulatory T cell MyD88/RORγt pathway to suppress food allergy
Azza Abdel-Gadir1,2, Emmanuel Stephen-Victor1,2, Georg K Gerber3
1Division of Immunology, Boston Children's Hospital, Boston, MA, USA.
Abstract:
The role of dysbiosis in food allergy (FA) remains unclear. We found that dysbiotic fecal microbiota in FA infants evolved compositionally over time and failed to protect against FA in mice. Infants and mice with FA had decreased IgA and increased IgE binding to fecal bacteria, indicative of a broader breakdown of oral tolerance than hitherto appreciated. Therapy with Clostridiales species impacted by dysbiosis, either as a consortium or as monotherapy with Subdoligranulum variabile, suppressed FA in mice as did a separate immunomodulatory Bacteroidales consortium. Bacteriotherapy induced expression by regulatory T (Treg) cells of the transcription factor ROR-γt in a MyD88-dependent manner, which was deficient in FA infants and mice and ineffectively induced by their microbiota. Deletion of Myd88 or Rorc in Treg cells abrogated protection by bacteriotherapy. Thus, commensals activate a MyD88/ROR-γt pathway in nascent Treg cells to protect against FA, while dysbiosis impairs this regulatory response to promote disease.
Related Concept Videos
Cis-regulatory Sequences
Cis-regulatory Sequences
Forces Acting on Chromosomes
Microtubules and motor proteins exert two types of forces on...
C4 Pathway and CAM
C4 Pathway
The C4 pathway is used by plants such as...
Global Regulatory Systems
Direct-Acting Cholinergic Agonists: Pharmacokinetics

