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Updated: Jan 23, 2026

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
Published on: October 20, 2019
New microsatellites for the Atacama Desert endemic Balsamocarpon brevifolium (Fabaceae)
Gonzalo Ravest1, Pedro León-Lobos1, Carlos Aguirre1
1Instituto de Investigaciones Agropecuarias (INIA) La Platina Santa Rosa 11610 Santiago Chile.
Premise:
Algarrobilla (Balsamocarpon brevifolium, Fabaceae) is an endemic xerophytic shrub restricted to the Atacama Desert in northern Chile. Extensive utilization of the region for coal production has endangered this species. Conservation efforts are underway, with genetic diversity analyses being key to the restoration of these populations.
Methods And Results:
Fifteen new microsatellite markers were developed for B. brevifolium and used to analyze three populations from the Atacama and Coquimbo regions in Chile. Microsatellites were highly polymorphic, with an average of 5.77 alleles per marker and an average level of expected heterozygosity of 0.72. These markers were evaluated and cross-amplified on two related species (Senna cumingii and Caesalpinia angulata) with partial success.
Conclusions:
The development of this set of markers permits an extensive study of B. brevifolium populations for conservation purposes.
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