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Updated: Jan 23, 2026

Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Evaluation of the Candida sp. 99-125 Lipase Positional Selectivity for 1,3-Diolein Synthesis
Yanhong Bi1, Zhangqun Duan2, Wenjing Zhang1
1School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, China.
Abstract:
In this study, comparative experiments were carried out to investigate the positional selectivity of Candida sp. 99-125 lipase in preparing 1,3-diolein by using medium engineering strategy. The results indicated that the diolein yield was markedly enhanced from 56.5% to 86.7% with increasing logP values of the solvents, while the selectivity of the examined lipase for the sn-1 over the sn-2 hydroxyl of glycerol was decreased, thus leading to a reduced 1,3-diolein to 1,2-diolein ratio. To evaluate the possibility of industrial enzymatic production of 1,3-diolein, larger-scale experiments were assessed. After being used repeatedly for eight batches, the diolein content reached 95.1%, while the 1,3-diolein to 1,2-diolein ratio was 7:1 following purification. Results of the kg level experiments significantly demonstrated the practicability of the enzymatic process and the efficiency of the purification strategy for the product.
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