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Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
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Focused Ultrasound Effects on Osteosarcoma Cell Lines
Valentina Agnese1, Viviana Costa1, Gian Luca Scoarughi2
1IRCCS Istituto Ortopedico Rizzoli, Via di Barbiano, 1/10 - 40136 Bologna, Italy.
Biomed Research International
|June 26, 2019
Summary
Magnetic Resonance guided Focused Ultrasound (MRgFUS) impacts osteosarcoma cells differently based on acoustic intensity. Lower MRgFUS doses may select for heterogeneous, immature tumor cells, potentially preserving bone tumors.
Area of Science:
- Oncology
- Biophysics
- Biomedical Engineering
Background:
- Magnetic Resonance guided Focused Ultrasound (MRgFUS) is an effective non-invasive therapeutic modality for various tumor ablations.
- While MRgFUS is established for ablative treatments, its biological effects on osteosarcoma cells at sub-lethal acoustic intensities remain under-investigated.
- Understanding these effects is crucial for optimizing MRgFUS applications in bone cancer treatment.
Purpose of the Study:
- To investigate the biological impact of MRgFUS on osteosarcoma cell lines (Mg-63 and Saos-2) at varying acoustic intensities (15.5-49 W/cm²).
- To evaluate the effects of acoustic energy dissipation outside the focal zone on cell viability and osteogenic differentiation.
- To determine the cellular response to sub-lethal acoustic power levels relevant to MRgFUS treatments.
Main Methods:
- Exposure of Mg-63 and Saos-2 osteosarcoma cells to MRgFUS at acoustic intensities ranging from 15.5 to 49 W/cm².
- Assessment of cell viability and osteogenic differentiation at 24 hours, 7 days, and 14 days post-treatment.
- Analysis of acoustic energy dissipation effects from focal to 'far field' zones.
Main Results:
- MRgFUS treatment at higher intensities (49-38.9 W/cm²) led to decreased cell proliferation rates.
- Lower acoustic intensities (31.1-15.5 W/cm²) resulted in the selection of a heterogeneous subpopulation of immature, viable osteosarcoma cells.
- These findings suggest that sub-lethal acoustic intensities may preserve bone tumor cells, indicating a complex biological response to MRgFUS.
Conclusions:
- The biological effects of MRgFUS on osteosarcoma cells are intensity-dependent, with lower doses potentially promoting cell survival and heterogeneity.
- Acoustic energy dissipation outside the focal zone significantly influences cellular response, impacting viability and differentiation.
- Further research is needed to elucidate the mechanisms behind these responses and to refine MRgFUS protocols for effective osteosarcoma treatment.
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