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Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
Published on: June 26, 2010
Near-infrared fluorescent probe for selective detection of H2S and its application in living animals
Xilang Jin1, Sumei Zhao2, Ting Wang1
1School of Materials and Chemical Engineering, Xi'an Technological University, Xi'an, 710032, Shaanxi, China.
Abstract:
In this study, a novel near-infrared fluorescent off-on probe for H2S based on seminaphthorhodafluor fluorophore is designed and constructed, which could be used in detection with 121-fold (23-fold) fluorescent (absorbance) enhancement at 630 nm (572 nm), fast responsiveness (completed within 5 min), high sensitivity, and lower cellular autofluorescence interference. Based on these excellent optical properties, the probe was employed to monitor H2S in red wine samples with satisfactory results. Moreover, the probe was successfully applied for monitoring and imaging H2S quantitatively in Hela cells and live athymic nude mice, indicating its potential application in biological science.
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