Related Experiment Video
Updated: Jan 23, 2026

Nanopore DNA Sequencing for Metagenomic Soil Analysis
Published on: December 14, 2017
Metagenomic sequencing reveals microbial gene catalogue of phosphinothricin-utilized soils in South China
Jiali Lou1, Min Liu1, Jiali Gu2
1State Key Lab of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
Abstract:
In the work, metagenomic sequencing was conducted to investigate the microbial gene catalogue in two samples of phosphinothricin (PPT)-utilized soils from South China. The gene sets contained an overwhelming majority of prevalent microbial genes, and were largely shared between these two samples. Several genus with high abundance were shared, such as norank_d__Bacteria, Nitrososphaera, Candidatus_Nitrosotalea, Candidatus_Nitrosocosmicus, and Rhodanobacter. Bacitracin resistance genes (61.4%) were the most dominant antibiotic resistance genes in two samples, followed by multidrug resistance efflux pump (12.5%). A lot of common virulence factors with high abundance were found in two samples, such as Alginate, Capsule I, ClpC, FbpABC, and HitABC, many of which were used for the iron uptake system. Total 57 putative PPT acetyltransferase were annotated, and two of them were found to be novel putative acetyltransferases for acetylation and detoxification of PPT. In conclusion, the work revealed microbial gene catalogue of PPT-utilized soils and found two novel putative PPT acetyltransferases using metagenomics. The work facilitates the understanding of impact of PPT on complex microbial community structure and physiology resides in PPT-utilized soils. Moreover, two annotated PPT acetyltransferases show important potential for the development of transgenic herbicide-resistant crops.
More Related Videos
10:28Investigating the Relationship between Sea Surface Chlorophyll and Major Features of the South China Sea with Satellite Information
Published on: June 13, 2020
11:23Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples
Published on: December 22, 2014
Related Concept Videos
The Soil Ecosystem
Gene Regulation in Microbial Communities: Quorum Sensing
Gene Flow
Cis-regulatory Sequences
What is Gene Expression?
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Gene Families
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...