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Updated: Jan 23, 2026

Preparation of Binary and Ternary Deep Eutectic Systems
Published on: October 31, 2019
Hydrophobic deep eutectic solvent-based dispersive liquid-liquid microextraction for the simultaneous enantiomeric
Yongjing Liu1, Wen Xu1, Hua Zhang1
1Pharmacy College, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian Province, P. R. China.
Abstract:
In this study, a simple and effective method was developed for the enantiomeric analysis of five β-agonists (terbutaline, clorprenaline, tulobuterol, clenbuterol, and salbutamol) in water samples using deep eutectic solvent (DES) based dispersive liquid-liquid microextraction and chiral LC-MS. In such a framework, different kinds of hydrophobic DESs were tailored to examine their extraction ability for five β-agonists from aqueous sample. After an initial screening, the primary factors affecting the extraction recovery of DES based dispersive liquid-liquid microextraction, such as hydrogen-bond acceptor/hydrogen-bond donor ratio, DES volume, type and volume of disperser solvent and so on, were investigated and optimized. Finally, the established method was validated and found to be linear, precise, and accurate. The method was successfully applied to analyze the five β-agonists in water samples, which will help better understand the behavior of individual enantiomer and make accurate risk assessment on the ecosystem.
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