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Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells
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Hypoxia-Preconditioned Adipose-Derived Endothelial Progenitor Cells Promote Bladder Augmentation
Feng Zhao1, Liuhua Zhou1, Zhongle Xu1
1Department of Urology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
Tissue Engineering. Part A
|June 27, 2019
Summary
Hypoxic preconditioning of adipose-derived endothelial progenitor cells (hp-ADEPCs) significantly enhanced vascularization and smooth muscle regeneration in tissue-engineered bladders. This approach improved bladder function and offers a promising solution for bladder reconstruction challenges.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Stem Cell Biology
Background:
- Bladder reconstruction faces challenges with insufficient vascularization in engineered tissues.
- Short-term hypoxic preconditioning can enhance stem cell angiogenic effects.
- Adipose-derived endothelial progenitor cells (ADEPCs) possess angiogenic properties.
Purpose of the Study:
- To evaluate the effect of hypoxia-preconditioned ADEPCs (hp-ADEPCs) on vascularization and smooth muscle regeneration in tissue-engineered bladders.
- To investigate the mechanism by which hp-ADEPCs enhance vascularization.
- To assess the functional recovery of engineered bladders using hp-ADEPCs and bladder acellular matrix (BAM).
Main Methods:
- Rats underwent partial cystectomy and augmentation with hp-ADEPC-BAM, ADEPC-BAM, or BAM.
- Histological and functional assessments were performed at 1 and 3 months post-surgery.
- Mechanisms of hp-ADEPCs on vascularization were investigated via immunohistochemistry and cell assays.
Main Results:
- hp-ADEPC-BAM significantly promoted urothelium, blood vessel, smooth muscle, and nerve regeneration.
- Animals receiving hp-ADEPC-BAM showed improved bladder compliance and micturition patterns.
- hp-ADEPCs enhanced ERK phosphorylation, HIF-1α expression, and secretion of VEGF and bFGF, boosting endothelial cell migration and angiogenesis.
Conclusions:
- Hypoxic preconditioning of ADEPCs enhances their angiogenic potential.
- The combination of hp-ADEPCs and BAM promotes vascularization and functional recovery in tissue-engineered bladders.
- This study presents a novel strategy for improving bladder tissue engineering outcomes.
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