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Updated: Jan 23, 2026

Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia
Published on: August 14, 2017
Cloning, expression and activity of ATP-binding protein in Bacillus thuringiensis toxicity modulation against Aedes
Guo-Hui Zhao1, Jian-Nan Liu1, Xiao-Hua Hu1
1State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & School of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Background:
Bacillus thuringiensis israelensis (Bti) is a widely used mosquitocidal microbial pesticide due to its high toxicity. ATP-binding proteins (ABP) are prevalently detected in insects and are related to reaction against Bti toxins. However, the function of ABP in mosquito biocontrol is little known, especially in Aedes aegypti. Therefore, this study aimed to clarify the function of ABP in Ae. aegypti against Bti toxin.
Results:
Aedes aegypti ABP (GenBank: XM_001661856.2) was cloned, expressed and purified in this study. Far-western blotting and ELISA were also carried out to confirm the interaction between ABP and Cry11Aa. A bioassay of Cry11Aa was performed both in the presence and absence of ABP, which showed that the mortality of Ae. aegypti is increased with an increase in ABP.
Conclusions:
Our results suggest that ABP in Ae. aegypti can modulate the toxicity of Cry11Aa toxin to mosquitoes by binding to Bti toxin. This could not only enrich the mechanism of Bt toxin, but also provide more data for the biocontrol of this transmission vector.
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