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A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene
Published on: April 1, 2019
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Stability of thromboxane in blood samples
Helga Helgadóttir1,2, Ísleifur Ólafsson2, Karl Andersen3
1Faculty of Pharmaceutical Sciences, University of Iceland, Reykjavik, Iceland.
Vascular Health and Risk Management
|June 27, 2019
Summary
Blood collection tube type and storage time significantly impact thromboxane B2 levels. Citrate tubes show increased thromboxane B2 over time, while EDTA tubes maintain stability, affecting diagnostic accuracy.
Area of Science:
- Biochemistry
- Clinical Pathology
- Hematology
Background:
- Venous blood collection can activate platelets, leading to thromboxane A2 release.
- Platelet activation post-collection may yield inaccurate thromboxane A2 measurements.
Purpose of the Study:
- To determine if thromboxane A2 synthesis continues in blood tubes after collection.
- To assess the impact of sample handling on thromboxane B2 stability.
Main Methods:
- Blood samples collected in citrate and EDTA vials from 10 individuals.
- Plasma aliquots stored in polypropylene tubes at 0, 30, and 120 minutes.
- Thromboxane B2 measured via enzyme immunoassay; some citrate samples stored with indomethacin.
Main Results:
- Thromboxane B2 increased by 400% in citrate tubes stored in polypropylene after 120 minutes.
- Indomethacin in citrate tubes reduced the increase to 200% over 120 minutes.
- EDTA tubes showed a 10% decrease in thromboxane B2 over 120 minutes.
Conclusions:
- Sample storage conditions critically affect thromboxane B2 stability.
- Vial type and time to centrifugation significantly influence results.
- Proper sample handling is essential for accurate thromboxane B2 assessment.
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