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Updated: Jan 23, 2026

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
Published on: January 17, 2014
Construction and Isolation of Recombinant Vaccinia Virus Expressing Fluorescent Proteins
N Bishara Marzook1, Timothy P Newsome2
1The University of Sydney, School of Life and Environmental Sciences, Sydney, NSW, Australia.
Abstract:
Vaccinia virus recombinants that express fluorescent proteins have a variety of applications such as the identification of infected cells, efficient screening for genetically modified strains, and molecular characterization of virus replication and spread. The detection of fluorescent proteins and viral-fluorescent fusion proteins by fluorescence microscopy is noninvasive and can be used to describe protein localization in live cells and track the intracellular movement of virus particles. This chapter describes a number of approaches for the construction of plasmids and subsequent generation and isolation of fluorescent recombinant viruses.
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