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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Viruses with RNA Genomes01:29

Viruses with RNA Genomes

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RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
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What are Viruses?00:50

What are Viruses?

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Overview
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RNA Editing02:23

RNA Editing

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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Related Experiment Video

Updated: Jan 23, 2026

CRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus AAV and 2-Cell Embryo Electroporation
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Vaccinia Virus Genome Editing Using CRISPR.

Carmela Di Gioia1, Ming Yuan1, Yaohe Wang2

  • 1Barts Cancer Institute, Queen Mary University, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|June 27, 2019
PubMed
Summary
This summary is machine-generated.

CRISPR/Cas9 gene editing precisely modifies genomes in living cells. This study demonstrates its efficient use for targeted transgene insertion into the vaccinia virus genome.

Keywords:
CRISPRCas9EditingGenomeHomologous recombinationVaccinia virus

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Area of Science:

  • Molecular Biology
  • Genetics
  • Virology

Background:

  • CRISPR/Cas9 is a powerful tool for precise genome editing.
  • Modifying viral genomes is crucial for research and therapeutic applications.

Purpose of the Study:

  • To demonstrate the efficient application of CRISPR/Cas9 for vaccinia virus genome editing.
  • To enable targeted insertion of transgenes into the vaccinia virus genome.

Main Methods:

  • Utilized the CRISPR/Cas9 system for targeted genome modification.
  • Applied the method to the vaccinia virus, a large DNA virus.

Main Results:

  • Achieved efficient editing of the vaccinia virus genome.
  • Successfully inserted transgene(s) at a specific targeted site within the genome.

Conclusions:

  • CRISPR/Cas9 is an effective tool for engineering the vaccinia virus.
  • This method facilitates the precise genetic modification of vaccinia virus for future applications.