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Updated: Jan 23, 2026

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 1
Published on: April 8, 2009
Growth and Purification of Vaccinia Virus Stocks for MPM Imaging
Glennys V Reynoso1, John P Shannon1, Jeffrey L Americo2
1Viral Immunity and Pathogenesis Unit, Laboratory of Clinical Immunology and Microbiology (LCIM), National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD, USA.
Abstract:
This chapter provides methods for the propagation, purification, and titration of vaccinia virus (VACV) and the highly attenuated strain-modified vaccinia Ankara (MVA). Additionally, we provide information on VACV recombinants we have used for intravital imaging with multiphoton excitation.
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