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Related Concept Videos

Properties of Enantiomers and Optical Activity02:24

Properties of Enantiomers and Optical Activity

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It is essential to understand the difference between chiral and achiral interactions and the implications thereof in optical activity and their applications. Just as our feet, which are chiral, interact uniquely with chiral objects, such as a pair of shoes, but identically with achiral socks, enantiomers of a molecule exhibit different properties only when they interact with other chiral media. An example of a significant implication from this facet is the phenomenon known as optical activity,...
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Imaging Biological Samples with Optical Microscopy01:18

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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Development of the Sexual Organs in the Embryo and Fetus01:15

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Development of the reproductive organs in an embryo starts from a bipotential state. This means the early embryo can develop either male or female reproductive organs. The formation of these organs begins with the growth of gonadal ridges that arise from the intermediate mesoderm during the fifth week of development.
Near the gonadal ridges, two duct systems are present: the mesonephric ducts (Wolffian ducts) and paramesonephric ducts (Müllerian ducts). These ducts form the basis for the...
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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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Seed Structure and Early Development of the Sporophyte02:33

Seed Structure and Early Development of the Sporophyte

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Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo.
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The Angiosperm Life Cycle02:39

The Angiosperm Life Cycle

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Plants have a life cycle split between two multicellular stages: a haploid stage—with cells containing one set of chromosomes—and a diploid stage—with cells containing two sets of chromosomes. The haploid stage is the gamete-producing gametophyte, and the diploid stage is the spore-producing sporophyte.
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Related Experiment Video

Updated: Jan 23, 2026

Viability Assays for Cells in Culture
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Viability Assays for Cells in Culture

Published on: January 20, 2014

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Biodynamic optical assay for embryo viability.

Zhe Li1, Natalie Ehmke2, Ilka M Lorenzo2

  • 1Purdue University, Department of Physics and Astronomy, West Lafayette, Indiana, United States.

Journal of Biomedical Optics
|June 27, 2019
PubMed
Summary
This summary is machine-generated.

A novel biodynamic microscope (BDM) assesses early embryo viability by measuring metabolic activity. This label-free method accurately predicts developmental potential, offering a faster alternative to traditional assays in assisted reproduction.

Area of Science:

  • Biotechnology
  • Microscopy
  • Reproductive Biology

Background:

  • Assessing early embryo viability is crucial for assisted reproductive technology (ART).
Keywords:
coherent opticsdigital holographydynamic light scatteringembryo viability

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  • Current metabolic assays are often invasive and time-consuming.
  • Parthenogenetic embryos provide a model for studying early developmental potential.