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Modulation of cPKCγ on Synapsin-Ia/b-Specific Phosphorylation Sites in the Developing Visual Cortex of Mice

Tao Fu1, Jing Wang1, Yichao Ding1

  • 1Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Science Key Laboratory, Beijing, China.

Investigative Ophthalmology & Visual Science
|June 27, 2019
PubMed
Abstract

Insights

Synapsin-Ia/b protein levels and phosphorylation at Ser9 and 603 are crucial for mouse visual cortex development. Conventional protein kinase C gamma (cPKCγ) significantly modulates these phosphorylation changes.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Developmental Biology

Background:

  • Synapsins are key synaptic vesicle-associated proteins involved in regulating neurotransmitter release.
  • Visual cortical plasticity is essential for sensory processing and is influenced by dynamic molecular changes during development.

Purpose of the Study:

  • To investigate the dynamic changes in total protein expression and conventional protein kinase C gamma (cPKCγ)-modulated phosphorylation of synapsin-Ia/b.
  • To explore the role of synapsin-Ia/b in the developing visual cortex of mice.

Main Methods:

  • Western blot analysis was used to quantify total and phosphorylated synapsin-Ia/b levels at specific serine sites (Ser9, 549, 603).
  • Experiments utilized wild-type (cPKCγ+/+) and knockout (cPKCγ-/-) mice at various developmental stages (postnatal days 7-60).

Main Results:

  • Total synapsin-Ia/b protein levels increased significantly from P14 to P60 in the visual cortex, unaffected by cPKCγ knockout.
  • Phosphorylation levels at Ser9 and 603 significantly increased early in development (P21-P28) and decreased later (P35-P60).
  • cPKCγ knockout significantly inhibited both the increase and decrease of P-Ser9 and 603 synapsin-Ia/b levels, while P-Ser549 remained unchanged.

Conclusions:

  • Both the expression levels and cPKCγ-modulated phosphorylation status of synapsin-Ia/b at Ser9 and 603 are critical for mouse visual cortex development.
  • cPKCγ plays a significant role in regulating the phosphorylation dynamics of synapsin-Ia/b during visual cortex maturation.

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