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Area of Science:

  • Developmental biology
  • Gastroenterology
  • Comparative medicine

Background:

  • Preterm infants face unique health challenges due to organ immaturity.
  • Mouse models are vital for studying immature intestinal diseases, such as necrotizing enterocolitis (NEC).
  • A gap exists in understanding the direct comparison of intestinal developmental stages between mice and humans.

Purpose of the Study:

  • To establish developmental stage equivalency between the immature murine and human intestine.
  • To provide a framework for selecting appropriate mouse ages for modeling human intestinal development and disease.

Main Methods:

  • Harvested murine small intestines from birth to P28 (postnatal day).
  • Obtained human fetal and term-infant small intestine samples (17-23 weeks gestation and term).
  • Quantified intestinal cell types and marker gene expression in both species.

Main Results:

  • Murine and human intestinal development show similar trends over time.
  • Murine intestine before P10 mirrors human fetal intestine before viability.
  • P14 murine intestine aligns with 22-23 weeks gestation human intestine; P28 murine intestine matches term human intestine.

Conclusions:

  • C57BL/6J mice are a suitable model for the human immature intestine.
  • The selected age of the mouse is a critical determinant for successful model development.