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Characteristics of Artificial Immunogens Containing Peptide Mimotopes of HIV-1 Epitopes Recognized by Monoclonal

N S Shcherbakova1, A N Chikaev2, A P Rudometov3

  • 1State Research Center of Virology and Biotechnology Vector, Federal Service on Surveillance for Consumer Rights Protection and Human Well-Being (Rospotrebnadzor), Kol'tsovo, Novosibirsk region, Russia. shherbakova_ns@vector.nsc.ru.

Bulletin of Experimental Biology and Medicine
|June 28, 2019
PubMed

The paper describes construction of TBI-based recombinant proteins TBI-2F5 and TBI-2G12 that contain peptide mimotopes of HIV-1 epitopes recognized by broadly neutralizing antibodies 2F5 and 2G12, respectively. The capacity of the immunogens to induce neutralizing antibodies was evaluated. The sera of BALB/c mice immunized with recombinant proteins TBI, TBI-2F5, and TBI-2G12 neutralized HIV-1 env-pseudoviruses. Moreover, pooled serum from mice immunized with TBI-2F5 and TBI-2G12 neutralized env-pseudoviruses of HIV-1 subtype B more effectively than individual sera.

Keywords:
HIV-1HIV-1 immunogenmonoclonal antibodies 2F5 and 2G12phage display

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