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A Highly Versatile Expression System for the Production of Multiply Phosphorylated Proteins

Phillip Zhu1, Philip R Gafken2, Ryan A Mehl1

  • 1Oregon State University , Department of Biochemistry and Biophysics, 2011 Agricultural and Life Sciences , Corvallis , Oregon 97331 , United States.

ACS Chemical Biology
|June 28, 2019
PubMed
Summary

We developed a new platform, pSer-3.1G, for efficient phosphoserine incorporation into proteins using genetic code expansion. This system overcomes previous limitations, enabling high yields of pure, multiphosphorylated proteins for research.

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