Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chromosome abnormalities in myelofibrosis.

G Castoldi1, A Cuneo, P Tomasi

  • 1Department of Internal Medicine, University of Ferrara, Italia.

Acta Haematologica
|January 1, 1987
PubMed
Summary

Cytogenetic analysis of myelofibrosis with myeloid metaplasia (MMM) revealed common chromosomal abnormalities, particularly involving chromosome 3, suggesting a link to abnormal platelet production (thrombopoiesis).

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Thoracic insufficiency syndrome].

Acta ortopedica mexicana·2021
Same author

Head down tilt 15° in experimental intracerebral hemorrhage: a randomized noninferiority safety trial.

European journal of neurology·2020
Same author

Genetic landscape of ultra-stable chronic lymphocytic leukemia patients.

Annals of oncology : official journal of the European Society for Medical Oncology·2018
Same author

NAFLD/NASH in patients with type 2 diabetes and related treatment options.

Journal of endocrinological investigation·2017
Same author

Refined karyotype-based prognostic stratification of chronic lymphocytic leukemia with a low- and very-low-risk genetic profile.

Leukemia·2017
Same author

Management of CLL in the elderly.

Annals of oncology : official journal of the European Society for Medical Oncology·2016

Area of Science:

  • Hematology
  • Cytogenetics
  • Oncology

Background:

  • Myelofibrosis with myeloid metaplasia (MMM) is a chronic myeloproliferative neoplasm characterized by bone marrow fibrosis and extramedullary hematopoiesis.
  • Megakaryocytes, the cells responsible for platelet production, play a crucial role in the pathophysiology of MMM.

Purpose of the Study:

  • To investigate the cytogenetic abnormalities in megakaryocyte-enriched cell fractions from patients with MMM.
  • To explore the potential relationship between specific chromosomal alterations and dysplastic thrombopoiesis in MMM.

Main Methods:

  • Cytogenetic analysis was performed on megakaryocyte-enriched cell fractions.
  • Cells were isolated from blood, bone marrow, and ascitic fluid of 12 MMM patients using discontinuous Percoll gradients.

Main Results:

  • Common chromosomal abnormalities involved chromosomes 1, 3, 7, 8, 11, 13, 21, and 22.
  • No specific chromosome rearrangements were consistently found across different tissues.
  • A 3q abnormality in polyploid metaphases and a 3q paracentric inversion were observed in two separate patients.

Conclusions:

  • The study suggests a close relationship between chromosome 3 involvement and dysplastic thrombopoiesis in MMM.
  • Further research is warranted to elucidate the specific mechanisms linking these chromosomal abnormalities to platelet production defects.

Related Experiment Videos