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Updated: Jan 22, 2026

Utilizing Soil Density Fractionation to Separate Distinct Soil Carbon Pools
Published on: December 16, 2022
Characterization of marine bacterial carbonic anhydrase and their CO2 sequestration abilities based on a soil
Panchami Jaya1, Vinod Kumar Nathan1,2, Parvathi Ammini1
1Regional Centre, National Institute of Oceanography-CSIR , Cochin , India.
Abstract:
The novel technology of biological carbon sequestration using microbial enzymes have numerous advantages over conventional sequestration strategies. In the present study, extracellular carbonic anhydrase (CA) producing bacteria were isolated from water samples in the Arabian Sea, India. A potential isolate, Bacillus safensis isolate AS-75 was identified based on 16S rDNA sequence analysis. The culture conditions suitable for CA production were 32 °C incubation temperature with 4% NaCl and 10 mM Zn supplementation. Experimental optimization of culture conditions enhanced enzyme activity to 265 U mL-1. CA specific gene was characterized and based on the analysis, the CA of B. safensis isolate AS-75 was a leucine (11.3%) with α-helices as the dominant component in its secondary structure. Based on soil microcosm studies, CA could sequester CO2 by 95.4% ± 0.11% in sterilized soil with enzyme microcosm. Hence, the application of enzyme was found to be more effective in removing CO2.
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