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Updated: Jan 22, 2026
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Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
Published on: December 4, 2017
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Single-Cell High-Throughput Technologies in Cerebrospinal Fluid Research and Diagnostics
Tobias V Lanz1,2, Anne-Katrin Pröbstel3,4, Iris Mildenberger1
1Department of Neurology, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
Frontiers in Immunology
|June 28, 2019
Summary
High-throughput single-cell technologies are revolutionizing biomedical research and clinical pathology. This review updates single-cell proteomic and genomic methods for cerebrospinal fluid analysis, discussing future directions.
Area of Science:
- Biomedical Research
- Clinical Pathology
- Proteomics
- Genomics
Background:
- High-throughput single-cell technologies are increasingly vital in biomedical research.
- These methods show significant promise for clinical pathology applications, particularly with liquid and solid biopsies.
Purpose of the Study:
- To review current single-cell proteomic and genomic technologies for cerebrospinal fluid (CSF) research.
- To discuss the potential of these methods in CSF diagnostics and identify future research directions.
Main Methods:
- Review of high-throughput proteomic techniques: flow cytometry and mass cytometry.
- Analysis of genomic approaches: immune cell repertoire and single-cell transcriptomics.
- Critical evaluation of existing single-cell methods in CSF studies.
Main Results:
- Single-cell proteomic and genomic technologies offer powerful tools for CSF analysis.
- These methods enable detailed characterization of cell types within CSF samples.
- Current technologies provide a foundation for advancing CSF diagnostics.
Conclusions:
- Single-cell technologies are poised to transform cerebrospinal fluid research and diagnostics.
- Further development and application of these methods will enhance our understanding of neurological diseases.
- Future research should focus on refining high-throughput techniques for routine clinical use.
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