Soil mixture composition alters Arabidopsis susceptibility to Pseudomonas syringae infection

Pseudomonas syringae is a gram-negative bacterial pathogen that causes disease on more than 100 different plant species, including the model plant Arabidopsis thaliana. Dissection of the Arabidopsis thaliana-Pseudomonas syringae pathosystem has identified many factors that contribute to successful infection or immunity, including the genetics of the host, the genetics of the pathogen, and the environment. Environmental factors that contribute to a successful interaction can include temperature, light, and the circadian clock, as well as the soil environment. As silicon-amended Resilience soil is advertised to enhance plant health, we sought to examine the extent to which this soil might affect the behavior of the A. thaliana-P. syringae model pathosystem and to characterize the mechanisms through which these effects may occur. We found that plants grown in Si-amended Resilience soil displayed enhanced resistance to bacteria compared to plants grown in non-Si-amended Sunshine soil, and salicylic acid biosynthesis and signaling were not required for resistance. Although silicon has been shown to contribute to broad-spectrum resistance, our data indicate that silicon is not the direct cause of enhanced resistance and that the Si-amended Resilience soil has additional properties that modulate plant resistance. Our work demonstrates the importance of environmental factors, such as soil in modulating interactions between the plant and foliar pathogens, and highlights the significance of careful annotation of the environmental conditions under which plant-pathogen interactions are studied.