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Related Experiment Video

Updated: Jan 22, 2026

Lateral Root Inducible System in Arabidopsis and Maize

Lateral Root Inducible System in Arabidopsis and Maize

Published on: January 14, 2016

Development of an activation tagging system for maize.

John P Davies¹, Vaka S Reddy^1,2, Xing L Liu¹

¹Dow AgroSciences Indianapolis Indiana.

|June 28, 2019

Summary

We developed a novel maize activation tagging system using the Enhancer/Suppressor (En/Spm) transposon. This tool distributes transcriptional enhancers to discover gene function and identify agriculturally relevant traits.

Keywords:

activation tagging enhancer insertional mutagenesis transposable elements

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Scalable Transfection of Maize Mesophyll Protoplasts

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Published on: June 23, 2023

Area of Science:

Plant genetics
Molecular biology
Genomics

Background:

Activation tagging is crucial for plant gene discovery.
Existing methods require improvement for efficiency and broader application.

Purpose of the Study:

To develop a novel transposable element-based activation tagging system for maize.
To enable efficient genome-wide distribution of transcriptional enhancers.

Main Methods:

Utilized the Enhancer/Suppressor (En/Spm) transposon system.
Engineered a novel activation tagging element with multiple Sugar Cane Bacilliform Virus enhancers (SCBV-enhancer) and an AAD1 selectable marker.
Incorporated an engineered seed color marker for detecting transposon excision events (somatic and germinal).

Main Results:

Successfully developed and demonstrated a functional transposable element system for maize activation tagging.
Showcased germinal excision events with re-integration into non-linked genomic locations.
Observed sustained transposon activity over three generations with high germinal excision rates in T2 generation lines.

Conclusions:

The developed system provides a powerful tool for generating large-scale activation-tagged maize populations.
Facilitates the screening of these populations for agriculturally relevant phenotypes.
Advances functional genomics research in maize.