Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Animal and Plant Cell Structure01:30

Animal and Plant Cell Structure

47.4K
Animal and plant cells not only differ in their structure, function, and mode of nutrition but also in how they reproduce, specialize, and organize into complex structures.
Cell Division
Though both plant and animal cells divide by mitosis (for non-gametic cells) and meiosis (for gametic cells), they differ in the specifics of this process. Unlike animal cells, plant cells lack centrosomes — an organelle responsible for organizing the spindle fibers and segregating the chromosomes during...
47.4K
Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals01:17

Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals

3.4K
Ideally, an unpaired electron shows a single peak in the EPR spectrum due to the transition between the two spin energy states. However, coupling interactions can occur between the spins of the unpaired electron and any neighboring spin-active nuclei. This hyperfine coupling results in hyperfine splitting, where the EPR signal is split into multiplets. The signals split into 2nI + 1 peaks, where n is the number of equivalent nuclei and I is the nuclear spin. These splitting patterns provide...
3.4K
Tumor Progression02:07

Tumor Progression

7.2K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
7.2K
Structures of Solids02:22

Structures of Solids

17.5K
Solids in which the atoms, ions, or molecules are arranged in a definite repeating pattern are known as crystalline solids. Metals and ionic compounds typically form ordered, crystalline solids. A crystalline solid has a precise melting temperature because each atom or molecule of the same type is held in place with the same forces or energy. Amorphous solids or non-crystalline solids (or, sometimes, glasses) which lack an ordered internal structure and are randomly arranged. Substances that...
17.5K
What are Cells?01:07

What are Cells?

197.6K
Cells are the smallest and basic units of life, whether it is a single cell that forms the entire organism, e.g., in a bacterium or trillions of them, e.g., in humans. No matter what organism a cell is a part of, they share specific characteristics.
Basic Characteristics of Cells
A living cell has a plasma membrane, a bilayer of lipids that separates the aqueous solution inside the cell called the cytoplasm from the outside environment.
Furthermore, a living cell possesses genetic information...
197.6K
Additional Subnuclear Structures02:10

Additional Subnuclear Structures

5.3K
The eukaryotic nucleus is a double membrane-bound organelle that contains nearly all of the cell’s genetic material in the form of chromosomes. It is rightly called the “brain” of the cell as it shoulders the responsibility of responding to various physiological processes, stress, altered metabolic conditions, and other cellular signals. 
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles,...
5.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The C-terminal intrinsically disordered region of a fungal lytic polysaccharide monooxygenase binds copper and displays anti-fungal properties.

International journal of biological macromolecules·2026
Same author

Fluorinated Biradicals for <sup>19</sup>F Magic-Angle Spinning Dynamic Nuclear Polarization-Enhanced NMR Spectroscopy.

Journal of the American Chemical Society·2026
Same author

High Potential Isoindoline-Based Nitroxides Posolytes for Aqueous Organic Redox Flow Batteries.

ChemSusChem·2026
Same author

Hidden protein disorder: Deciphering the structural organisation and dynamics of a non-canonical CP12 from the diatom Thalassiosira pseudonana.

The FEBS journal·2025
Same author

Studies of the membrane-bound flavocytochrome MsrQ flavin mononucleotide (FMN)-binding site reveal an unexpected ubiquinone cofactor.

The FEBS journal·2025
Same author

Systematic Evaluation of Polarizing Agents for Dynamic Nuclear Polarization Enhanced NMR.

Angewandte Chemie (International ed. in English)·2025

Related Experiment Video

Updated: Jan 22, 2026

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
11:19

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

11.0K

In-Cell EPR: Progress towards Structural Studies Inside Cells.

Alessio Bonucci1, Olivier Ouari2, Bruno Guigliarelli3

  • 1Magnetic Resonance Center, CERM, University of Florence, 50019, Sesto Fiorentino, Italy.

Chembiochem : a European Journal of Chemical Biology
|June 28, 2019
PubMed
Summary

Investigating biomolecules inside cells using site-directed spin labeling (SDSL) electron paramagnetic resonance (EPR) spectroscopy offers unique insights. This review highlights advances and challenges in applying in-cell EPR for structural biology.

Keywords:
EPR spectroscopySDSLin-cell spectroscopyspin labelsstructural biology

More Related Videos

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment
10:46

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

Published on: March 16, 2018

8.7K
Studying Interactions between Myeloid Cells and CAR T Cells In Vitro and In Vivo
05:14

Studying Interactions between Myeloid Cells and CAR T Cells In Vitro and In Vivo

Published on: July 25, 2025

809

Related Experiment Videos

Last Updated: Jan 22, 2026

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
11:19

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

11.0K
In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment
10:46

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

Published on: March 16, 2018

8.7K
Studying Interactions between Myeloid Cells and CAR T Cells In Vitro and In Vivo
05:14

Studying Interactions between Myeloid Cells and CAR T Cells In Vitro and In Vivo

Published on: July 25, 2025

809

Area of Science:

  • Structural biology
  • Biophysics
  • Cellular imaging

Background:

  • Understanding biomolecular structure and dynamics within the native cellular environment is crucial.
  • In vitro methods struggle to fully replicate complex intracellular conditions.
  • Investigating biomolecules directly inside cells is gaining significant interest.

Purpose of the Study:

  • To review the major advances in in-cell electron paramagnetic resonance (EPR) spectroscopy.
  • To summarize the capabilities of site-directed spin labeling (SDSL) coupled with EPR for in-cell studies.
  • To discuss the current challenges and future directions of in-cell EPR approaches.

Main Methods:

  • Site-directed spin labeling (SDSL) of biomolecules.
  • Electron paramagnetic resonance (EPR) spectroscopy.
  • Application of in-cell EPR techniques in both bacterial and eukaryotic cells.

Main Results:

  • Successful application of in-cell EPR spectroscopy in various cellular systems.
  • SDSL-EPR provides unique advantages for studying protein structure and dynamics in situ.
  • Demonstrated feasibility of capturing biomolecular behavior within the native cellular milieu.

Conclusions:

  • In-cell EPR, particularly with SDSL, is a powerful technique for structural biology.
  • Significant progress has been made in applying EPR spectroscopy directly within living cells.
  • Further development is needed to overcome existing challenges for broader adoption.