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Updated: Jan 22, 2026

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells
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Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells

Published on: May 2, 2017

8.0K

The miR-1204 regulates apoptosis in NSCLC cells by targeting DEK

Zhen Qian1, Juan Yang1, Huanhuan Liu1

  • 1College of Life Sciences and Bio-engineering, Beijing Jiaotong University, Beijing 100044, China.

Folia Histochemica Et Cytobiologica
|June 28, 2019
PubMed
Abstract

Insights

MicroRNA-1204 (miR-1204) induces apoptosis in non-small cell lung cancer (NSCLC) by inhibiting the DEK oncogene. This process involves regulating Bcl-2 and Bax proteins and activating the caspase 9/3 pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Non-small cell lung cancer (NSCLC) is a leading cause of cancer-related mortality.
  • The DEK oncogene plays a role in cancer progression.
  • MicroRNAs (miRNAs) are key regulators of gene expression with implications in cancer.

Purpose of the Study:

  • To investigate the effect of miR-1204 on DEK oncogene expression in NSCLC.
  • To elucidate the molecular mechanisms underlying miR-1204's action in NSCLC cells.

Main Methods:

  • Transfection of miR-1204 mimics and inhibitors into NSCLC cell lines (A549 and SPC).
  • Analysis of DEK mRNA levels, cell viability, apoptosis rate, cell morphology, and caspase activity.
  • Assessment of apoptosis-related proteins Bcl-2 and Bax expression.

Main Results:

  • miR-1204 overexpression down-regulated DEK mRNA levels in NSCLC cells.
  • miR-1204 overexpression significantly increased apoptosis rates and induced characteristic morphological changes.
  • Expression of Bcl-2 was decreased, while Bax expression was increased following miR-1204 overexpression.
  • DEK inhibition by miR-1204 involved the mitochondria-related caspase 9/3 pathway.

Conclusions:

  • miR-1204 induces apoptosis in NSCLC cells by inhibiting DEK expression.
  • The mechanism involves down-regulation of Bcl-2 and up-regulation of Bax.
  • Mitochondria-related caspase 9/3 pathway mediates the apoptosis induced by miR-1204 and DEK inhibition.
Keywords:
A549 cellsBaxBcl-2DEKNSCLCSPC cellsapoptosiscaspase-3caspase-9miR-1204

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