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Updated: Jan 22, 2026

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions
Published on: June 14, 2016
Quantification of Drug Molecules in Live Single Cells Using the Single-Probe Mass Spectrometry Technique
Ning Pan1, Shawna J Standke1, Naga Rama Kothapalli1
1Department of Chemistry and Biochemistry , University of Oklahoma , Norman , Oklahoma 73019 , United States.
Abstract:
Analyzing cellular constituents on the single-cell level through mass spectrometry (MS) allows for a wide range of compounds to be studied simultaneously. However, there is a need for quantitative single-cell mass spectrometry (qSCMS) methods to fully characterize drug efficacy from individual cells within cell populations. In this study, qSCMS experiments were carried out using the Single-probe MS technique. The method was successfully used to perform rapid absolute quantifications of the anticancer drug irinotecan in individual mammalian cancer cells under ambient conditions in real time. Traditional liquid chromatography/mass spectrometry (LC/MS) quantifications of irinotecan in cell lysate samples were used to compare the results from Single-probe qSCMS. This technique showcases heterogeneity of drug efficacy on the single-cell level.
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