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Nitrogen atoms, present in all proteins and DNA, are recycled between abiotic and biotic components of the ecosystem. However, the primary form of nitrogen on Earth is nitrogen gas, which cannot be used by most animals and plants. Thus, nitrogen gas must first be converted into a usable form by nitrogen-fixing bacteria before it can be cycled through other living organisms. The use of nitrogen-containing fertilizers and animal waste products in human agriculture has greatly influenced the...
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Pilot relaying is a type of differential protection used in power systems. It compares electrical quantities at the terminals of equipment via a communication channel instead of direct relay interconnection. This method is essential for transmission lines where the terminals are far apart, typically up to 80 km for lines with 69 to 115 kV ratings. Four types of communication channels are used for pilot relaying:
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Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of  ammonia, ammonium ions, nitrate, nitrite, or  nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
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Updated: Jan 22, 2026

Mesocosm-Scale Constructed Wetland Design for Wastewater Treatment
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Nitrous oxide emissions from pilot scale three-stage constructed wetlands with variable nitrogen loading.

Shunan Zhang1, Feng Liu1, Pei Luo1

  • 1Key Laboratory of Agro-ecological Processes in Subtropical Regions, Changsha Research Station for Agricultural & Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan 410125, PR China.

Bioresource Technology
|June 28, 2019
PubMed
Summary

Constructed wetlands effectively remove nitrogen from swine wastewater. However, high nitrogen levels can increase nitrous oxide (N2O) emissions, linked to nitrate and N2O reductase gene abundance.

Keywords:
Constructed wetlandN(2)O emissionSwine wastewaterTotal nitrogennosZ gene

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Area of Science:

  • Environmental Engineering
  • Water Treatment Technologies
  • Microbial Ecology

Background:

  • Swine wastewater presents significant nitrogen pollution challenges.
  • Constructed wetlands are a promising biological treatment method.
  • Nitrous oxide (N2O) is a potent greenhouse gas emitted during nitrogen removal processes.

Purpose of the Study:

  • To investigate nitrous oxide (N2O) emissions from pilot-scale surface flow constructed wetlands (SFCWs) treating swine wastewater.
  • To determine the relationship between nitrogen loading, N2O emissions, and microbial gene abundance.
  • To provide insights for optimizing constructed wetland design for nitrogen removal and greenhouse gas mitigation.

Main Methods:

  • Utilized three-stage surface flow constructed wetlands (SFCWs) at a pilot scale.
  • Monitored nitrogen levels (total nitrogen, nitrate nitrogen) in swine wastewater.
  • Quantified nitrous oxide (N2O) emissions and measured the abundance of the N2O reductase (nosZ) gene.

Main Results:

  • SFCWs achieved high total nitrogen (TN) removal efficiencies (84.6-97.1%).
  • N2O emissions and nitrate nitrogen (NO3--N) concentration peaked at approximately 100 mg N/L TN.
  • N2O emissions positively correlated with NO3--N concentration (p < 0.001) and nosZ gene abundance (p < 0.05).
  • TN loading significantly affected nosZ gene abundance.

Conclusions:

  • Nitrogen removal in constructed wetlands can lead to significant N2O emissions.
  • N2O emissions are influenced by nitrogen loading, nitrate concentrations, and microbial N2O reductase activity.
  • Optimizing pollution loading is crucial for balancing nitrogen removal efficiency and minimizing N2O emissions in constructed wetland design.