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Molecular cloning and DNA sequence analysis of the human guanine nucleotide-binding protein Go alpha
1Biological Response Modifiers Program, National Cancer Institute-Frederick Cancer Research Facility, MD 21701.
Biochemical and Biophysical Research Communications
|January 29, 1988
Abstract:
The nucleotide sequence of human Go alpha was determined from a partial human brain cDNA clone and the sequence of the first two 5' coding exons of a human genomic Go alpha clone. Comparison of this sequence with bovine and rat Go alpha shows greater than 90% homology at the nucleotide and deduced amino acid level. There is 100% identity at the amino acid level for the cholera and pertussis toxin-catalyzed ADP ribosylation sites, the putative guanine nucleotide binding, and the GTP hydrolysis sites.