Related Experiment Video
Updated: Jan 22, 2026

Mammosphere Formation Assay from Human Breast Cancer Tissues and Cell Lines
Published on: March 22, 2015
Data on SVCT2 transporter expression and localization in cancer cell lines and tissues
Francisco J Roa1, Eduardo Peña1, Eveling Inostroza1
1Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Barrio Universitario s/n, PO Box 160C, Concepción, Chile.
Abstract:
The data presented in this article are related to the research paper entitled "Increased expression of mitochondrial sodium-coupled ascorbic acid transporter-2 (mitSVCT2) as a central feature in breast cancer", available in Free Radical Biology and Medicine Journal [1]. In this article, we examined the SVCT2 transporter expression in various breast cancer cell lines using RT-PCR and Western blot assays. In addition, we analyzed the subcellular localization of SVCT2 by immunofluorescence colocalization assays and cellular fractionation experiments. Finally, an analysis of different cancer tissue microarrays immunostained for SVCT2 and imaged by The Human Protein Atlas (https://www.proteinatlas.org) is presented.
Related Concept Videos
Cell Lines
Regulated mRNA Transport
Plant Cells and Tissues
Phloem and Sugar Transport
Facilitated Transport
Primary Active Transport

