Immuno-localization of ESCRT Proteins in Virus-Infected Cells by Fluorescence and Electron Microscopy
Keisuke Tabata1, Atsuki Nara2, Hiroko Omori3
1Department of Infectious Diseases, Molecular Virology, Heidelberg University, Heidelberg, Germany.
Abstract:
Many enveloped viruses utilize the cellular ESCRT pathway for budding, even flaviviruses, which form viral particles inside replication organelles derived from the endoplasmic reticulum (ER). In this section, we introduce methods for detecting several ESCRT subunit proteins in virus-infected cells by immunofluorescence microscopy and immunoelectron microscopy (immuno-EM). We also introduce a new method; correlative light microscopy and electron microscopy (CLEM), which allows the observation of target structures with both high-resolution EM and fluorescence labeling.
More Related Videos
11:45The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells
Published on: February 27, 2020
12:28Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
Published on: November 10, 2017
Related Concept Videos
Overview of Electron Microscopy
Scanning Electron Microscopy
Fundamental Principles
Accelerated...
Transmission Electron Microscopy
Immunogold Electron Microscopy
Cryo-electron Microscopy
Preparation of Samples for Electron Microscopy
