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A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
Published on: January 25, 2018
Transient Expression of ESCRT Components in Arabidopsis Root Cell Suspension Culture-Derived Protoplasts
Marie-Kristin Nagel1, Karin Vogel1, Erika Isono2
1Plant Physiology and Biochemistry, Department of Biology, University of Konstanz, Konstanz, Germany.
Abstract:
Localization studies are important to understand the function of diverse proteins. The endosomal trafficking pathway is very complex, and a lot of proteins function in this pathway, primarily the endosomal sorting complexes required for transport (ESCRTs). Some of the ESCRT-related proteins or mutant variants cannot be stably expressed in planta due to the toxicity of their expression. Therefore, a transient expression system is necessary to study their function. Transient expression in protoplasts from Arabidopsis root cell-derived culture serves as a fast and reliable method for the expression and cell biological and biochemical analyses of otherwise toxic constructs.
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