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Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Induced Pluripotent Stem Cells01:06

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Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Embryonic Stem Cells00:58

Embryonic Stem Cells

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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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Updated: Jan 22, 2026

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
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Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

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CRISPR Base Editing in Induced Pluripotent Stem Cells.

Ya-Ju Chang1,2, Christine L Xu1,2, Xuan Cui1,2

  • 1Department of Ophthalmology, Columbia University, New York, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 29, 2019
PubMed
Summary
This summary is machine-generated.

CRISPR base editing in induced pluripotent stem cells (iPSCs) precisely modifies single DNA bases. This advanced gene editing technique offers efficient and accurate genetic modifications for biotechnology and disease research.

Keywords:
Base editingCas9Precise gene editingTarget-AIDiPS cells

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Area of Science:

  • Stem Cell Biology
  • Gene Editing Technologies

Background:

  • Induced pluripotent stem cells (iPSPs) hold significant promise for disease modeling and regenerative medicine.
  • Advancements in gene transduction and editing technologies are enhancing the capabilities of iPSCs.
  • Cas9-cytidine deaminases offer a method for introducing specific single-base mutations.

Purpose of the Study:

  • To describe the application of CRISPR base editing in iPSCs for precise genomic modifications.
  • To highlight the efficiency and accuracy of this gene editing approach.

Main Methods:

  • Utilizing CRISPR-Cas9 base editing systems for targeted C→T or G→A transitions.
  • Applying base editing techniques to iPSCs for precise genomic locus modification.

Main Results:

  • Demonstration of highly efficient and accurate gene modifications in iPSCs.
  • Successful application of base editing for precise genome editing.

Conclusions:

  • CRISPR base editing in iPSCs is a powerful tool for biotechnology and therapeutic applications.
  • This technique facilitates the investigation of disease mechanisms linked to specific mutations.