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Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
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Signal amplification in flow cytometry for cell surface antigen analysis.

Takeshi Mori1, Yoshiki Katayama1

  • 1Faculty of Engineering, Department of Chemistry and Biochemistry, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka, Japan.

Journal of Biochemistry
|June 29, 2019
PubMed
Summary

Signal amplification systems enhance flow cytometry

Keywords:
cell surface antigenflow cytometryfluorimetryimmunofluorescencesignal amplification

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Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Cell surface antigens are crucial for understanding cell function and lineage.
  • Flow cytometry is a key technique for analyzing cell surface antigens.
  • Current flow cytometry methods lack sensitivity for detecting many important antigens.

Purpose of the Study:

  • To explore signal amplification strategies for improving flow cytometry sensitivity.
  • To evaluate methods for enhancing the detection of cell surface antigens.

Main Methods:

  • Investigated signal enhancing systems for flow cytometry.
  • Compared strategies like using bright fluorophores versus enzymatic signal amplification.
  • Assessed the impact of amplification methods on antibody functional characteristics.

Main Results:

  • Enzymatic signal amplification offers a practical and efficient approach to enhance sensitivity.
  • Signal amplification is essential for practical flow cytometry analysis of cell surface antigens.
  • While fluorophore-based methods can improve sensitivity, they may alter antibody function.

Conclusions:

  • Enzymatic signal amplification is a promising strategy for analyzing cell surface antigens by flow cytometry.
  • This approach enhances sensitivity without compromising antibody activity.
  • Further development of enzymatic methods is warranted despite existing drawbacks.