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Updated: Jan 22, 2026

An Objective and Reproducible Test of Olfactory Learning and Discrimination in Mice
Published on: March 22, 2018
Olfactory discrimination in disorders of consciousness: A new sniff protocol
Davide Sattin1, Maria Grazia Bruzzone2, Stefania Ferraro2
1Neurology, Public Health and Disability Unit, Coma Research Center, Fondazione IRCCS Istituto Neurologico Carlo Besta, Milan, Italy.
Background:
The identification of salient stimuli useful for rehabilitation purposes is important in patients with disorders of consciousness (DOC): among these, olfactory stimuli might play an important role due to the functional coupling between olfactory and emotional processing. However, a high percentage of post brain injury patients present anosmia.
Aims Of The Study:
The aim of this pilot research is to present an innovative approach to test olfactory functions at the bedside using four selected odors in patients with DOC.
Methods:
Sixteen patients with DOC were tested with two assessment techniques the new olfactory discrimination protocol (ODP) and a functional magnetic resonance imaging paradigm to evaluate olfactory neural process. The Frequentist and Bayesian methods were used to analyze reliability properties of the new tool.
Results:
Analysis showed a good agreement between assessment techniques and a substantial test-retest reliability of the ODP. Cohen's Ks were equal to 0.814 (95% CI = 0.471, 1) and 0.607 (0.118; 1) respectively, using the Frequentist approach, while they were 0.762 (95% HPD = 0.470; 0.966) and 0.650 (0.320; 0.913) with the Bayesian approach in the 11 patients analyzed.
Conclusions:
Despite the limits of this preliminary research, the ODP can be useful for clinicians for the preliminary assessment of the olfactory discrimination in patients with DOC.
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