Design and development of a "Y-shaped" microbial consortium capable of simultaneously utilizing biomass sugars for...

Microbial production of chemicals from lignocellulosic biomass is usually hampered by the low efficiency of the simultaneous utilization of C5 and C6 sugars. In nature, this is not a problem because different C5- and C6-utilizing microorganisms cooperate. Nevertheless, the diverse metabolism of microorganisms in nature makes it difficult to synchronize the utilization of biomass sugars toward a specific goal. To address this problem, we sought to develop a novel microbial consortium that can mimic nature's ability of efficiently use biomass sugars, while synchronizing this capability toward a useful goal to maximize the power of nature and engineering. Starting from a completely chromosomally engineered butanol hyper-producing Escherichia coli strain that we developed previously, we developed a consortium comprising two E. coli strains with nearly identical genomic backgrounds, thus creating a "Y-shaped" consortium with two different "heads" (using xylose or glucose) but the same "body" (from glycolysis to butanol production). This "Y-shaped" chimeric consortium achieved the most efficient butanol production from mixed sugars reported to date, by equally efficient and orthogonal consumption of C5 and C6 sugars. Furthermore, we show that the consortium structure is not only adaptive to environmental perturbations, but can be arbitrarily changed to simultaneously utilize C5/C6 sugars in different ratio. The design and development of such a "Y-shaped" chimeric consortium provides a novel approach to address the need for simultaneous efficient utilization of different biomass sugars for the production of useful chemicals.