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Updated: Jan 22, 2026

Measuring the Kinetics of mRNA Transcription in Single Living Cells
Published on: August 25, 2011
Towards mapping the 3D genome through high speed single-molecule tracking of functional transcription factors in
Adam J M Wollman1, Erik G Hedlund1, Sviatlana Shashkova1
1Biological Physical Science Institute, Departments of Physics and Biology, University of York, YO10 5DD York, UK.
Abstract:
How genomic DNA is organized in the nucleus is a long-standing question. We describe a single-molecule bioimaging method utilizing super-localization precision coupled to fully quantitative image analysis tools, towards determining snapshots of parts of the 3D genome architecture of model eukaryote budding yeast Saccharomyces cerevisiae with exceptional millisecond time resolution. We employ astigmatism imaging to enable robust extraction of 3D position data on genomically encoded fluorescent protein reporters that bind to DNA. Our relatively straightforward method enables snippets of 3D architectures of likely single genome conformations to be resolved captured via DNA-sequence specific binding proteins in single functional living cells.
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