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Panel of reference strains for evaluating serologic reagents used to identify gonococci
G M Evins1, N E Pigott, J S Knapp
1Center for Infectious Diseases, Atlanta, Georgia 30333.
Journal of Clinical Microbiology
|February 1, 1988
Summary
A diverse panel of Neisseria gonorrhoeae strains was created to test serologic reagents. GonoGen demonstrated superior accuracy, correctly identifying all gonococcal and nongonococcal strains tested.
Area of Science:
- Microbiology
- Immunology
- Diagnostic Development
Background:
- Accurate serologic diagnosis of Neisseria gonorrhoeae is crucial for public health.
- Existing diagnostic reagents require rigorous evaluation against diverse bacterial strains.
Purpose of the Study:
- To develop and utilize a comprehensive strain panel for evaluating Neisseria gonorrhoeae serologic reagents.
- To assess the performance of commercially available diagnostic kits.
Main Methods:
- A panel of 52 gonococcal and 20 nongonococcal strains, antigenically diverse and from various sources, was assembled.
- Three commercial kits (GonoGen, Phadebact monoclonal antibody, Phadebact absorbed antiserum) were tested against the strain panel.
Main Results:
- GonoGen correctly identified all 52 gonococcal and 20 nongonococcal strains.
- Phadebact absorbed antiserum identified 47/48 gonococcal strains but cross-reacted with 2 nongonococcal strains.
- Phadebact monoclonal antibody identified all gonococcal strains but showed cross-reactivity with 8 and trace reactions with 4 nongonococcal strains.
Conclusions:
- GonoGen exhibited excellent specificity and sensitivity in this evaluation.
- The developed strain panel is a valuable tool for assessing Neisseria gonorrhoeae serologic diagnostic performance.
- Further refinement of Phadebact reagents may be needed to improve specificity.