Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein Rou. A human IgA hybrid.

E Mihaesco1, M C Gendron, N Congy

  • 1Laboratory of Immunochemistry and Immunopathology, Hôpital Saint-Louis, Paris, France.

Journal of Immunology (Baltimore, Md. : 1950)
|February 15, 1988
PubMed
Summary

Human immunoglobulin A2 (IgA2) myeloma protein Rou exhibits a hybrid heavy chain. This suggests a genetic recombination event between alpha 1 and alpha 2 genes in its B-cell lineage.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Conformation as therapeutic target in the prionoses and other neurodegenerative conditions.

Methods in molecular medicine·2011
Same author

Use of fluorescent probes to assess the early sulfhydryl depletion and oxidative stress induced by mechlorethamine in human bronchial epithelial cells.

Toxicology in vitro : an international journal published in association with BIBRA·2010
Same author

Expression of BRI2 mRNA and protein in normal human brain and familial British dementia: its relevance to the pathogenesis of disease.

Neuropathology and applied neurobiology·2008
Same author

Molecular chaperons, amyloid and preamyloid lesions in the BRI2 gene-related dementias: a morphological study.

Neuropathology and applied neurobiology·2006
Same author

Genetic alterations of the BRI2 gene: familial British and Danish dementias.

Brain pathology (Zurich, Switzerland)·2006
Same author

Chromosome 13 dementias.

Cellular and molecular life sciences : CMLS·2005

Area of Science:

  • Immunogenetics
  • Protein Chemistry
  • Molecular Biology

Background:

  • Immunoglobulin A (IgA) is a critical antibody isotype involved in mucosal immunity.
  • Human IgA exists in two subclasses, IgA1 and IgA2, with distinct structural and functional properties.
  • Myeloma proteins provide valuable models for studying immunoglobulin structure and gene expression.

Purpose of the Study:

  • To characterize the structural basis of the human IgA2 myeloma protein Rou.
  • To investigate the genetic origin of the unique heavy chain composition of Protein Rou.
  • To elucidate the molecular mechanism underlying the formation of hybrid immunoglobulin heavy chains.

Main Methods:

  • Partial amino acid sequencing of the immunoglobulin heavy chain.
  • Comparative sequence analysis of different immunoglobulin domains.
  • Identification of structural homologies and disulfide bond patterns.

Main Results:

  • Protein Rou possesses a human IgA2 myeloma protein structure with the A2m(2) isoallotype marker.
  • Its heavy chain exhibits a hybrid structure: hinge and CH2 domains homologous to IgA2, while CH1 and CH3 domains are homologous to IgA1.
  • The CH1 domain contains a heavy-light chain disulfide bond characteristic of IgA1.

Conclusions:

  • The heavy chain of Protein Rou is a hybrid molecule resulting from a genetic recombination event between IgA1 and IgA2 genes.
  • This recombination occurred between exon 1 of the alpha 1 gene and the hinge exon of the alpha 2 gene.
  • The identified recombination site is located between positions 222-223 of the alpha-chain, providing insights into immunoglobulin gene evolution.

Related Experiment Videos