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Macrophage requirements of PPD-induced blastogenic factor production by T and B lymphocytes in the guinea-pig
An attempt has been made to characterize the cell type which releases blastogenic factor(s) (BF) on purified protein derivative (PPD) stimulation in complete Freund's adjuvant (CFA)-sensitized guinea-pig system and, further, to examine whether macrophages (Mphi) are required for antigen activation of T or B lymphocytes to produce this lymphokine. Both T and B lymphocyte populations from guinea-pigs 2-3 weeks after CFA sensitization (early-stage T and B cells) failed to produce significant amounts of BF in response to PPD, whereas both the populations from 10 to 14 weeks after sensitization (late-stage T and B cells) acquired the ability to produce BF by themselves. It is noteworthy that readded autologous Mphi not only functioned well to augment BF production by the late-stage T and B cells but also completely made up for the inability of the early-stage T and B cells to produce BF. From these data it is reasonable to conclude that in the guinea-pig sensitized T and B cells can both release BF on exposure to PPD and that Mphi either reconstitute or augment the BF synthetic response of T and B cells.
An attempt has been made to characterize the cell type which releases blastogenic factor(s) (BF) on purified protein derivative (PPD) stimulation in complete Freund's adjuvant (CFA)-sensitized guinea-pig system and, further, to examine whether macrophages (Mphi) are required for antigen activation of T or B lymphocytes to produce this lymphokine. Both T and B lymphocyte populations from guinea-pigs 2-3 weeks after CFA sensitization (early-stage T and B cells) failed to produce significant amounts of BF in response to PPD, whereas both the populations from 10 to 14 weeks after sensitization (late-stage T and B cells) acquired the ability to produce BF by themselves. It is noteworthy that readded autologous Mphi not only functioned well to augment BF production by the late-stage T and B cells but also completely made up for the inability of the early-stage T and B cells to produce BF. From these data it is reasonable to conclude that in the guinea-pig sensitized T and B cells can both release BF on exposure to PPD and that Mphi either reconstitute or augment the BF synthetic response of T and B cells.