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In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
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USP21 modulates Goosecoid function through deubiquitination.

Fuwei Liu1, Qian Fu1, Yunpeng Li1

  • 1State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Disease & Shaanxi Key Laboratory of Oral Diseases, Department of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, People's Republic of China.

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|June 30, 2019
PubMed
Summary
This summary is machine-generated.

The ubiquitin carboxyl-terminal hydrolase 21 (USP21) deubiquitylates Goosecoid (GSC), a key gene in craniofacial development. USP21 negatively regulates GSC activity, impacting cell functions.

Keywords:
DeubiquitinationGoosecoidSox6 reporter gene systemUSP21

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Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • Goosecoid (GSC) is a homeobox gene crucial for craniofacial development.
  • GSC activity is regulated by mono-ubiquitination, but the responsible deubiquitylase remains unknown.

Purpose of the Study:

  • To identify deubiquitylases regulating GSC expression.
  • To investigate the role of USP21 in GSC deubiquitination and function.

Main Methods:

  • Constructed recombinant plasmid pFlag-CMV-2-GSC.
  • Developed an SRY (sex-determining region Y)-box 6 (Sox6) reporter gene system.
  • Assessed USP21 interaction with GSC and its effect on Sox6 transcription and ATDC5 cell function.

Main Results:

  • Identified ubiquitin carboxyl-terminal hydrolase 21 (USP21) as a negative regulator of GSC deubiquitination.
  • USP21 interacts with GSC, promoting its deubiquitination without affecting protein stability.
  • USP21 influences ATDC5 cell viability, migration, and function via GSC modulation.

Conclusions:

  • USP21 modulates GSC function through deubiquitination.
  • This study identifies USP21 as a key regulator of GSC activity in craniofacial development.