Related Experiment Video
Updated: Jan 22, 2026
10:33
Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells
Published on: May 22, 2014
14.6K
Pluripotent stem cell-derived organogenesis in the rat model system
Masumi Hirabayashi1,2, Teppei Goto3, Shinichi Hochi4
1Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Higashiyama 5-1, Myodaiji-cho, Okazaki, Aichi, 444-8787, Japan. mhirarin@nips.ac.jp.
Transgenic Research
|June 30, 2019
Summary
Rats are ideal for regenerative medicine research, enabling organ regeneration through gene editing and stem cell therapies. This study highlights successful in vivo organ generation in rat models for transplantation advancements.
Area of Science:
- Biomedical Research
- Regenerative Medicine
- Developmental Biology
Background:
- Rats offer advantages for xenotransplantation research, including available stem cell lines and larger organs.
- Genome-editing tools and stem cell technology are revolutionizing regenerative medicine.
Purpose of the Study:
- To provide an overview of recent advancements in stem cell-derived organ regeneration using rat models.
- Focus on gene knock-out and blastocyst complementation techniques.
Main Methods:
- Gene knock-out of Pdx1, Foxn1, and Sall1 to create organ-deficient rat models.
- Microinjection of pluripotent stem cells into blastocyst-stage rat embryos for chimeric development.
Main Results:
- Successful generation of rat models lacking pancreas, thymus, and kidney.
- Development of chimeric rat offspring with stem cell-derived functional organs in vivo.
- Demonstrated ability to generate three-dimensional organs using pluripotent stem cells.
Conclusions:
- Stem cell-derived organ generation in vivo accelerates regenerative medicine and transplantation therapies.
- Ethical considerations and public consent are crucial for producing human organs in animals.
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