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Related Experiment Video

Updated: Jan 22, 2026

A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms
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Hormones modulate Candida vaginal isolates biofilm formation and decrease their susceptibility to azoles and hydrogen

Bruna Gonçalves1, Nuno Miguel Azevedo1, Mariana Henriques1

  • 1CEB, Center of Biological Engineering, LIBRO - "Laboratório de Investigação em Biofilmes Rosário Oliveira", University of Minho, Gualtar Campus, 4715-057 Braga, Portugal.

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|June 30, 2019
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Summary

Hormones like progesterone and estradiol enhance Candida glabrata

Keywords:
Candida sppbiofilmprogesteronevulvovaginal candidiasisβ-estradiol

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Area of Science:

  • Mycology
  • Infectious Diseases
  • Endocrinology

Background:

  • Vulvovaginal candidiasis (VVC) is a common infection caused by Candida species, increasingly involving antifungal-resistant Candida glabrata.
  • Hormonal fluctuations, particularly during pregnancy, are known to promote VVC, yet their direct impact on Candida cells, especially C. glabrata, remains understudied.
  • Candida species form biofilms, contributing to VVC pathogenesis and resistance to treatments.

Purpose of the Study:

  • To investigate the influence of progesterone and β-estradiol on biofilm formation and antifungal resistance in vaginal isolates of Candida albicans and Candida glabrata.
  • To analyze these effects under acidic conditions (pH 4), mimicking the vaginal environment, and at hormone concentrations relevant to the menstrual cycle and pregnancy.

Main Methods:

  • Culturing Candida albicans and Candida glabrata vaginal isolates.
  • Treating isolates with physiological concentrations of progesterone and β-estradiol.
  • Assessing biofilm formation, biomass, and filamentation.
  • Evaluating susceptibility to azole antifungals and hydrogen peroxide.

Main Results:

  • Hormone exposure reduced Candida albicans biofilm biomass by up to 65% and impaired filamentation.
  • Candida glabrata demonstrated high adaptability, with hormones showing no significant impact on its biofilm formation.
  • Hormones increased resistance of both Candida species to azoles and hydrogen peroxide, particularly at pregnancy-related concentrations.

Conclusions:

  • Hormones can act as environmental cues that protect Candida species from vaginal defenses and antifungal treatments.
  • Candida glabrata's adaptability to hormones suggests a significant role in VVC pathogenicity, especially during pregnancy.
  • Findings highlight potential implications for VVC treatment strategies, particularly for infections caused by C. glabrata.