Related Experiment Video
Updated: Jan 22, 2026

Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
Published on: September 18, 2016
Ap4A Regulates Directional Mobility and Antigen Presentation in Dendritic Cells
Shin La Shu1, Lakshmi Bhargavi Paruchuru2, Neil Quanwei Tay1
1Immunology Programme, Department of Microbiology, Centre for Life Sciences, National University of Singapore, #03-09, 28 Medical Drive, Singapore 117456, Singapore; NUS-HUJ-CREATE, 1 Create Way, Innovation Wing #03-09, Singapore 138602, Singapore.
Abstract:
The significance of intracellular Ap4A levels over immune activity of dendritic cells (DCs) has been studied in Nudt2fl/fl/CD11c-cre mice. The transgenic mice have been generated by crossing floxed NUDT2 gene mice with DC marker CD11c recombinase (cre) mice. The DCs derived from these mice have higher levels of Ap4A (≈30-fold) compared with those derived from Nudt2+/+ mice. Interestingly, the elevated Ap4A in DCs has led them to possess higher motility and lower directional variability. In addition, the DCs are able to enhance immune protection indicated by the higher cross-presentation of antigen and priming of CD8+ OT-I T cells. Overall, the study denotes prominent impact of Ap4A over the functionality of DCs. The Nudt2fl/fl/CD11c-cre mice could serve as a useful tool to study the influence of Ap4A in the critical immune mechanisms of DCs.
More Related Videos
18:08Interview: Glycolipid Antigen Presentation by CD1d and the Therapeutic Potential of NKT cell Activation
Published on: December 31, 2007
12:48Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation
Published on: August 21, 2017
Related Concept Videos
Self-Presentation
Self-Presentation: Self-Monitoring and Self-Handicapping
Strategies of Self-Presentation I: Strategic Self-Presentation
Processes of Self-Presentation
Antigen Presenting Cells
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...
pH Regulation in Cells
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...