Effect of Lentinan on Peyer's patch structure and function in an immunosuppressed mouse model

Area of Science:

• Immunology
• Gastroenterology
• Pharmacology

Background:

• Lentinan (LNT), a polysaccharide from *Lentinus edodes*, is known for immune-stimulating properties.
• Limited research exists on LNT's biological activities after oral administration.
• The intestinal mucosal immune system's response to LNT requires further investigation.

Purpose of the Study:

• To investigate the effects of orally administered LNT on the intestinal mucosal immune system of immunosuppressed mice.
• To evaluate LNT's impact on Peyer's patches (PPs), lymphocytes, and immune cell function.
• To assess LNT's influence on M-like cells and intestinal secretory immunoglobulin A (IgA).

Main Methods:

• Oral administration of LNT to immunosuppressed mice for 7 days.
• Analysis of Peyer's patch number, size, and lymphocyte proliferation.
• Determination of T and B lymphocyte percentages and T-cell activation.
• Assessment of M-like cell numbers, antigen transfer ability, and intestinal soluble IgA levels.

Main Results:

• LNT administration increased the number of PPs and lymphocytes within PPs.
• LNT maintained T and B lymphocyte percentages while upregulating T-cell activation.
• The number and antigen transfer ability of M-like cells were enhanced.
• Intestinal soluble IgA levels were significantly increased in LNT-treated mice.

Conclusions:

• Orally administered Lentinan effectively improves the intestinal mucosal immune status in immunosuppressed mice.
• LNT demonstrates potential as an immunomodulatory agent for enhancing gut immunity.
• These findings support LNT's role in bolstering mucosal defense mechanisms.