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Determination of Microbial Extracellular Enzyme Activity in Waters, Soils, and Sediments using High Throughput Microplate Assays
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An alternative methodology to determine 210Pb activity soil profiles.

M L Montes1, M G Rizzoto2, J Juri Ayub3

  • 1IFLP- CONICET- CCT La Plata, CC 67, Diagonal 113 y 64, 1900, La Plata, Argentina; Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina.

Journal of Environmental Radioactivity
|July 1, 2019
PubMed
Summary

This study introduces a new method for measuring unsupported 210Pb (lead-210) activity in soil, crucial for dating sediments and studying erosion. The technique bypasses the need for calibration sources, offering a viable alternative for laboratories lacking specialized equipment.

Keywords:
(210)Pb activityAlternative methodologyGamma rayUnsupported (210)Pb

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Area of Science:

  • Environmental Science
  • Geochemistry
  • Radiochemistry

Background:

  • Unsupported 210Pb (lead-210) is atmospheric and deposits on soil, valuable for sediment dating, erosion studies, and air mass analysis.
  • Determining 210Pb activity via gamma ray spectrometry (46.5 keV) is often hindered by the absence of an efficiency calibration curve, particularly without radioactive patron sources.

Purpose of the Study:

  • To present an alternative methodology for quantifying 210Pb activity in soil samples.
  • To validate this new method against conventional techniques, addressing the common challenge of unavailable calibration sources.

Main Methods:

  • The proposed method relies on activity definition and attenuation coefficient determination.
  • It assumes that soil layers below 25 cm are free of unsupported 210Pb, meaning 210Pb activity equals 226Ra activity at secular equilibrium.
  • The methodology was tested using soil samples from the La Plata region, Argentina.

Main Results:

  • The alternative methodology yielded comparable 210Pb activity values to the conventional method.
  • The results demonstrate the proposed procedure's effectiveness and reliability.

Conclusions:

  • The developed methodology provides a robust alternative for 210Pb activity determination when calibration sources are unavailable.
  • This approach enhances the accessibility of 210Pb dating and environmental studies in various laboratory settings.