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How should living entomological samples be stored?
V Bugelli1,2, C P Campobasso3, R Zehner4
1Department of Medicine and Health Sciences (DiMeS), University of Molise, Via De Sanctis, 86100, Campobasso, Italy. vale.buge@gmail.com.
Temporary storage of insect evidence significantly impacts survival and growth. Cold temperatures with oxygen and food supply are recommended for preserving forensic entomology samples, especially for blow fly larvae.
Area of Science:
- Forensic Entomology
- Entomology
- Insect Biology
Background:
- Proper handling of insect evidence is crucial in forensic entomology.
- Survival and growth rates of insect evidence can be affected by storage conditions.
Purpose of the Study:
- To investigate the impact of cooling and storage on insect evidence before laboratory analysis.
- To determine optimal conditions for preserving insect evidence, focusing on blow fly larvae.
Main Methods:
- Larval stages of Lucilia sericata and Calliphora vicina were subjected to different storage conditions (room temperature vs. refrigeration) for 24 hours.
- Simulated transport conditions involved storage at 6-8°C for 8 hours without air or food.
- Mortality rates, interim growth, and adult eclosion were assessed and compared to a control group.
Main Results:
- A 24-hour temporary storage halted larval growth, particularly in early stages.
- High mortality rates (up to 100%) were observed in third instar larvae stored without air supply.
- Oxygen supply significantly reduced mortality in L3 larvae of L. sericata.
Conclusions:
- Temporary storage significantly impacts insect evidence, necessitating careful handling.
- Cold storage with oxygen and food supply is recommended for preserving insect evidence.
- Rapid delivery and improved storage procedures, including air supply, are vital for last instar larvae.
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