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How should living entomological samples be stored?

V Bugelli1,2, C P Campobasso3, R Zehner4

  • 1Department of Medicine and Health Sciences (DiMeS), University of Molise, Via De Sanctis, 86100, Campobasso, Italy. vale.buge@gmail.com.

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Temporary storage of insect evidence significantly impacts survival and growth. Cold temperatures with oxygen and food supply are recommended for preserving forensic entomology samples, especially for blow fly larvae.

Keywords:
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Area of Science:

  • Forensic Entomology
  • Entomology
  • Insect Biology

Background:

  • Proper handling of insect evidence is crucial in forensic entomology.
  • Survival and growth rates of insect evidence can be affected by storage conditions.

Purpose of the Study:

  • To investigate the impact of cooling and storage on insect evidence before laboratory analysis.
  • To determine optimal conditions for preserving insect evidence, focusing on blow fly larvae.

Main Methods:

  • Larval stages of Lucilia sericata and Calliphora vicina were subjected to different storage conditions (room temperature vs. refrigeration) for 24 hours.
  • Simulated transport conditions involved storage at 6-8°C for 8 hours without air or food.
  • Mortality rates, interim growth, and adult eclosion were assessed and compared to a control group.

Main Results:

  • A 24-hour temporary storage halted larval growth, particularly in early stages.
  • High mortality rates (up to 100%) were observed in third instar larvae stored without air supply.
  • Oxygen supply significantly reduced mortality in L3 larvae of L. sericata.

Conclusions:

  • Temporary storage significantly impacts insect evidence, necessitating careful handling.
  • Cold storage with oxygen and food supply is recommended for preserving insect evidence.
  • Rapid delivery and improved storage procedures, including air supply, are vital for last instar larvae.